Hu, Yue and Pei, Wenqin and Hu, Ying and Li, Ping and Sun, Chen and Du, Jiawei and Zhang, Ying and Miao, Fengqin and Zhang, Aifeng and Shen, Yuqing and Zhang, Jianqiong (2020) MiR34a Regulates Neuronal MHC Class I Molecules and Promotes Primary Hippocampal Neuron Dendritic Growth and Branching. Frontiers in Cellular Neuroscience, 14. ISSN 1662-5102
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Abstract
In the immune system, Major Histocompatibility Complex class I (MHC-I) molecules are located on the surface of most nucleated cells in vertebrates where they mediate immune responses. Accumulating evidence indicates that MHC-I molecules are also expressed in the central nervous system (CNS) where they play important roles that are significantly different from their immune functions. Classical MHC-I molecules are temporally and spatially expressed in the developing and adult CNS, where they participate in the synaptic formation, remodeling and plasticity. Therefore, clarifying the regulation of MHC-I expression is necessary to develop an accurate understanding of its function in the CNS. Here, we show that microRNA 34a (miR34a), a brain enriched noncoding RNA, is temporally expressed in developing hippocampal neurons, and its expression is significantly increased after MHC-I protein abundance is decreased in the hippocampus. Computational algorithms identify putative miR34a target sites in the 3′UTR of MHC-I mRNA, and here we demonstrate direct targeting of miR34a to MHC-I mRNA using a dual-luciferase reporter assay system. MiR34a targeting can decrease constitutive MHC-I expression in both Neuro-2a neuroblastoma cells and primary hippocampal neurons. Finally, miR34a mediated reduction of MHC-I results in increased dendritic growth and branching in cultured hippocampal neurons. Taken together, our findings identify miR34a as a novel regulator of MHC-I for shaping neural morphology in developing hippocampal neurons.
Item Type: | Article |
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Subjects: | STM Digital Press > Medical Science |
Depositing User: | Unnamed user with email support@stmdigipress.com |
Date Deposited: | 20 May 2023 06:00 |
Last Modified: | 14 Sep 2024 04:17 |
URI: | http://publications.articalerewriter.com/id/eprint/882 |