Characterization of Learning, Motivation, and Visual Perception in Five Transgenic Mouse Lines Expressing GCaMP in Distinct Cell Populations

Groblewski, Peter A. and Ollerenshaw, Douglas R. and Kiggins, Justin T. and Garrett, Marina E. and Mochizuki, Chris and Casal, Linzy and Cross, Sissy and Mace, Kyla and Swapp, Jackie and Manavi, Sahar and Williams, Derric and Mihalas, Stefan and Olsen, Shawn R. (2020) Characterization of Learning, Motivation, and Visual Perception in Five Transgenic Mouse Lines Expressing GCaMP in Distinct Cell Populations. Frontiers in Behavioral Neuroscience, 14. ISSN 1662-5153

[thumbnail of pubmed-zip/versions/1/package-entries/fnbeh-14-00104/fnbeh-14-00104.pdf] Text
pubmed-zip/versions/1/package-entries/fnbeh-14-00104/fnbeh-14-00104.pdf - Published Version

Download (2MB)

Abstract

To study the mechanisms of perception and cognition, neural measurements must be made during behavior. A goal of the Allen Brain Observatory is to map the activity of distinct cortical cell classes underlying visual and behavioral processing. Here we describe standardized methodology for training head-fixed mice on a visual change detection task, and we use our paradigm to characterize learning and behavior of five GCaMP6-expressing transgenic lines. We used automated training procedures to facilitate comparisons across mice. Training times varied, but most transgenic mice learned the behavioral task. Motivation levels also varied across mice. To compare mice in similar motivational states we subdivided sessions into over-, under-, and optimally motivated periods. When motivated, the pattern of perceptual decisions were highly correlated across transgenic lines, although overall performance (d-prime) was lower in one line labeling somatostatin inhibitory cells. These results provide important context for using these mice to map neural activity underlying perception and behavior.

Item Type: Article
Subjects: STM Digital Press > Biological Science
Depositing User: Unnamed user with email support@stmdigipress.com
Date Deposited: 05 Jan 2023 10:37
Last Modified: 29 Apr 2024 07:47
URI: http://publications.articalerewriter.com/id/eprint/8

Actions (login required)

View Item
View Item