Monitoring the Maturation of Crambe Seeds Using X-ray Image Analysis

The application of modern and efficient techniques to access seed physical and physiological information has contributed to new advances in the agricultural sector. The objective of this study was to evaluate morphometric parameters obtained from the X-Ray analysis using crambe seeds harvested at different maturation stages and to relate them to seed physiological potential. Seeds harvested at different maturation stages were evaluated using X-Ray test, germination, first germination count, germination speed index, root protrusion velocity and accelerated aging. The Principal Components Analysis was applied to data collected. The results demonstrated that the X-Ray analysis allowed to visualize internal morphology of crambe seeds. Differences between seeds harvested at different maturation levels were observed. Correlations between physical variables were verified, such as seed area with germination (r = 0.96). The use of X-Ray analysis combined with semi-automated analysis is efficient to differentiate crambe seeds in different maturation stages. The morphometric parameters are related to the physiological quality of crambe seeds.