Michaelle, Chokki and Cheikna, Zongo and Durand, Dah-Nouvlessounon and Mihaela, Cudălbeanu and Pacôme, Noumavo and Ioana, Otilia Ghinea and Bianca, Furdui and Aly, Savadogo and Rodica, Mihaela Dinica and Lamine, Baba-Moussa and Farid, Baba-Moussa (2020) Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin. African Journal of Microbiology Research, 14 (8). pp. 426-435. ISSN 1996-0808
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Abstract
Momordica charantia and Morinda lucida are Benin’s pharmacopeia plants that are used traditionally for the treatment of infectious diseases. This study aims to investigate phytochemical profile and antimicrobial activity of both plants. The dried leaf powder is used for extraction with different solvents by ultrasonication (35 Hz) at room temperature for 2 h. TLC and the method based on coloring and precipitation differential reactions were used for preliminary screening. HPTLC analysis was performed on silica gel 60 F254, 20.0 X 10.0 cm HPTLC plates, with Toluen: Ethyl acetate: Formic Acid: Methanol (3:4:0.8:0.7 v/v) as a mobile phase. The antibacterial and antifungal activities were assessed in vitro by the method of macrodillution and solid medium agar diffusion. TLC analysis showed many spots which suggest that both of the plants extracts contain various secondary metabolites. HPTLC revealed the presence of Quercetine, cafeic acid and vanilic acid in the plants’ extract. M. charantia extracts have shown the largest inhibition diameters (25.00±0.00 mm) and inhibit more strains than M. lucida extracts. From all the tested strains, only P. aeruginosa was the most sensitive to M. charantia extracts with 50% bactericidal effect.
Item Type: | Article |
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Subjects: | STM Digital Press > Biological Science |
Depositing User: | Unnamed user with email support@stmdigipress.com |
Date Deposited: | 30 Mar 2023 07:55 |
Last Modified: | 28 Aug 2024 13:42 |
URI: | http://publications.articalerewriter.com/id/eprint/386 |