Study on the Plasmid and Genomic DNA Stability of DH5α Host System

Vengili, Laxmisai Dixitha and Sago, Phavethra and Kuncha, Yaswanth Kumar Reddy and Kolli, Venkata Suneela and Varugu, Lasya Sri and Mandadapu, Sree Lakshmi and Veeravilli, Suryanarayana and Vemuri, Praveen Kumar (2021) Study on the Plasmid and Genomic DNA Stability of DH5α Host System. Journal of Pharmaceutical Research International, 33 (60B). pp. 1325-1328. ISSN 2456-9119

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Abstract

Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media.

Item Type: Article
Subjects: STM Digital Press > Medical Science
Depositing User: Unnamed user with email support@stmdigipress.com
Date Deposited: 02 Mar 2023 09:56
Last Modified: 24 Jun 2024 05:11
URI: http://publications.articalerewriter.com/id/eprint/149

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