Nwaokorie, Francisca O. and Savage, Kofoworola O. and Fowora, Muinah A. and Ayanbadejo, Patricia O. and Ogunsola, Folasade T. and Coker, Akitoye O. (2013) Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria. British Microbiology Research Journal, 3 (4). pp. 492-500. ISSN 22310886
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Abstract
Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene.
Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010.
Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii.
Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum.
Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.
Item Type: | Article |
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Subjects: | STM Digital Press > Biological Science |
Depositing User: | Unnamed user with email support@stmdigipress.com |
Date Deposited: | 23 Jun 2023 06:28 |
Last Modified: | 26 Jun 2024 10:43 |
URI: | http://publications.articalerewriter.com/id/eprint/1195 |