Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria

Nwaokorie, Francisca O. and Savage, Kofoworola O. and Fowora, Muinah A. and Ayanbadejo, Patricia O. and Ogunsola, Folasade T. and Coker, Akitoye O. (2013) Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria. British Microbiology Research Journal, 3 (4). pp. 492-500. ISSN 22310886

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Abstract

Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene.
Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010.
Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii.
Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum.
Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.

Item Type: Article
Subjects: STM Digital Press > Biological Science
Depositing User: Unnamed user with email support@stmdigipress.com
Date Deposited: 23 Jun 2023 06:28
Last Modified: 26 Jun 2024 10:43
URI: http://publications.articalerewriter.com/id/eprint/1195

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