Barringtonia asiatica Seed Extract Induces G1 Cell Cycle Arrest in Saccharomyces cerevisiae and Exhibits Cytotoxicity in A2780 Human Ovarian Cancer Cells

Villanueva, Nilita Sirikit P. and Vicera, Carmela Vannette B. and Leonida, Sheeny Fane L. and Paderog, Melissa June and Sabido, Edna M. and Saludes, Jonel P. and Dalisay, Doralyn S. (2021) Barringtonia asiatica Seed Extract Induces G1 Cell Cycle Arrest in Saccharomyces cerevisiae and Exhibits Cytotoxicity in A2780 Human Ovarian Cancer Cells. Journal of Pharmaceutical Research International, 33 (53A). pp. 372-387. ISSN 2456-9119

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Abstract

Barringtonia asiatica, is traditionally used as a medicinal plant in the Philippines; unfortunately, there is limited evidence supporting its anticancer capability. Thus, our study investigated the cytotoxic effect of B. asiatica seed extract against ovarian carcinoma A2780 (ECACC 93112519) and Saccharomyces cerevisiae as a cancer model organism. Seeds of B. asiatica were freeze-dried and extracted with methanol. The seed extract was investigated for its effect on the cell cycle in S. cerevisiae by utilizing yeast budding experiment and imaging flow cytometry. Its cytotoxicity activity was also tested as well as a live/dead assay was conducted against ovarian cancer cells. Findings revealed the anticancer activity of B. asiatica and its capability to induce G1 cell cycle arrest in S. cerevisiae. Thus, B. asiatica seeds may serve as a potential source of natural compounds towards anticancer drug discovery.

Item Type: Article
Subjects: STM Digital Press > Medical Science
Depositing User: Unnamed user with email support@stmdigipress.com
Date Deposited: 29 Mar 2023 06:49
Last Modified: 09 Jul 2024 07:44
URI: http://publications.articalerewriter.com/id/eprint/104

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